Product Description
CleanPlex® Neurodegenerative Disorders1 Panel is a pre-designed and made-to-order multiplex PCR / amplicon-based targeted sequencing (NGS) assay designed to examine the germline variants or mutations across 32 genes associated with Alzheimer and Dementia Disease. The panel targets all the exonic regions of those genes and the flanking intronic sequences. Compatible with just 10 ng of DNA, sequencing-ready libraries can be prepared using a streamlined workflow in just 3 hours. The pre-designed panel is optimized in silico to deliver data with high on-target performance and high coverage uniformity to ensure efficient use of sequencing reads.
This product is made to order. Once we receive your order, we will synthesize the panel and the kit will contain CleanPlex Multiplex PCR Primers and CleanPlex Targeted Library Kit. CleanPlex Indexed PCR Primers and CleanMag® Magnetic Beads can be ordered separately to complete the workflow from input DNA to sequencing-ready NGS libraries.
Storage Temperature
Store at -20 °C.
For Research Use Only. Not for use in diagnostic procedures.
Gene List:
ABCA7, APOE, APP, C9ORF72, CHCHD10, CHMP2B, CSF1R, DCTN1, DNMT1, FUS, GRN, HNRNPA1, HNRNPA2B1, ITM2B, MAPT, NOTCH3, OPTN, PRNP, PSEN1, PSEN2, RNF216, SNCA, SNCB, SORL1, SQSTM1, TARDBP, TBK1, TREM2, TUBA4A, TYROBP, UBQLN2, VCP
References:
Bertram L. Next Generation Sequencing in Alzheimer’s Disease. Systems Biology of Alzheimer’s Disease. Methods in Molecular Biology, vol 1303 (2016).
Goldman JS, et al. Alzheimer’s Disease and Frontotemporal Dementia: The Current State of Genetics and Genetic Testing Since the Advent of Next-Generation Sequencing. Mol Diagn Ther 22, 505–513 (2018).
Koriath C, et al. Predictors for a dementia gene mutation based on gene-panel next-generation sequencing of a large dementia referral series. Mol Psychiatry (2018)
Rongioletti M, et al. Excess Copper in Alzheimer Disease but Not in Frontotemporal Lobar Degeneration: Next-Generation Sequencing Study of ATP7B Gene in Patients Typified by High Copper, American Journal of Clinical Pathology. (2018);150(1):S65–S66.
Gámez-Valero A,et al. Exploratory study on microRNA profiles from plasma-derived extracellular vesicles in Alzheimer’s disease and dementia with Lewy bodies. Transl Neurodegener 8, 31 (2019)
Goldman JS, et al. Alzheimer’s Disease and Frontotemporal Dementia: The Current State of Genetics and Genetic Testing Since the Advent of Next-Generation Sequencing. Mol Diagn Ther 22, 505–513 (2018).
Batoletti-Stella A, et al. Identification of rare genetic cariants in Italian patients with dementia by targeted gene sequencing. Neurobiology of Aging. 2018;66:e23-180.e31