CleanPlex®肺動脈高壓檢測板是一種預(yù)先設(shè)計(jì)和定制的，基于多重PCR /擴(kuò)增子的靶向測序（NGS）分析方法，旨在檢查與肺動脈高壓相關(guān)的10個基因的種系變異或突變。該小組針對這些基因的所有外顯子區(qū)域和側(cè)翼內(nèi)含子序列。僅需10 ng DNA即可兼容測序就緒的文庫，只需3個小時即可使用簡化的工作流程進(jìn)行準(zhǔn)備。預(yù)先設(shè)計(jì)的面板經(jīng)過計(jì)算機(jī)優(yōu)化，可提供具有高目標(biāo)性能和高覆蓋均勻性的數(shù)據(jù)，以確保有效利用測序讀數(shù)。
該產(chǎn)品是定做的。收到您的訂單后，我們將合成面板，該套件將包含CleanPlex Multiplex PCR引物和CleanPlex Targeted Library Kit。可以分別訂購CleanPlex索引PCR引物和CleanMag®磁珠，以完成從輸入DNA到可測序的NGS文庫的工作流程。
貯存溫度
儲存在-20°C。
僅供研究使用。不用于診斷過程。
Gene List: ACVRL1, BMPR1B, BMPR2, CAV1, EIF2AK4, ENG, GDF2, KCNA5, KCNK3, SMAD9 References: Morrell NW, et al. Genetics and genomics of pulmonary arterial hypertension. European Respiratory Journal Jan 2018, 1801899. Song J, et al. Identification of genetic defects in pulmonary arterial hypertension by a new gene panel diagnostic tool. Clin Sci (Lond) (2016) 130 (22): 2043-2052. Barozzi C, et al. A Combined Targeted and Whole Exome Sequencing Approach Identified Novel Candidate Genes Involved in Heritable Pulmonary Arterial Hypertension. Sci Rep 9, 753 (2019)
Gene List: ACVRL1, BMPR1B, BMPR2, CAV1, EIF2AK4, ENG, GDF2, KCNA5, KCNK3, SMAD9
References: Morrell NW, et al. Genetics and genomics of pulmonary arterial hypertension. European Respiratory Journal Jan 2018, 1801899.
Song J, et al. Identification of genetic defects in pulmonary arterial hypertension by a new gene panel diagnostic tool. Clin Sci (Lond) (2016) 130 (22): 2043-2052.
Barozzi C, et al. A Combined Targeted and Whole Exome Sequencing Approach Identified Novel Candidate Genes Involved in Heritable Pulmonary Arterial Hypertension. Sci Rep 9, 753 (2019)